39. Blum TR, Liu H, Packer MS, Xiong X, Lee PG, Zhang S, Richter M, Minasov G, Satchell KJF, Dong M*, Liu DR* (2021). Phase-assisted evolution of botulinum neurotoxin proteases with reprogrammed specificity. Science, 371:803

Science Perspective: https://science.sciencemag.org/content/371/6531/782.long

News reports: https://www.genengnews.com/news/evolved-proteases-selectively-cleave-target-sequences-unlock-potential-of-proteome-editing/



38. Miyashita S, Zhang J, Zhang S, Shoemaker CB, Dong M (2021). Delivery of single-domain antibodies into neurons using a chimeric toxin-based platform is therapeutic in mouse models of botulism. Science Translational Medicine, 13:eaaz4197.

Science In Depth: https://science.sciencemag.org/content/371/6525/113/tab-pdf

News reports: https://www.sciencedaily.com/releases/2021/01/210108142136.htm




37. Mansfield MJ, Tremblay BJM, Zeng J, Wei X, Hodgins H, Worley J, Bry L, Dong M*, Doxey AC*. (2020)  Phylogenomics of 8,839 Clostridioides difficile genomes reveals recombination-driven evolution and diversification of toxin A and B. PloS Pathogens, 16(12):e1009181

36. Tian S, Liu Y, Wu H, Liu H, Zeng J, Choi MY, Chen H, Gerhard R, Dong M (2020). Genome-wide CRISPR screen identifies semaphorin 6A and 6B as receptors for Paeniclostridium sordellii toxin TcsL. Cell Host and Microbe, 27:782-792. PMC7228847. 

35. Yin L, Masuyer G, Zhang S, Zhang J, Miyashita S, Burgin D, Lovelock L, Coker SF, Fu T, Stenmark P*, Dong M* (2020). Characterization of a membrane binding loop leads to engineering botulinum neurotoxin B with improved therapeutic efficacy. PloS Biology, 17;18(3):e3000618.

34. Martínez-Carranza M, Blasco P, Gustafsson R, Dong M, Berntsson RP, Widmalm G, Stenmark P (2020). Synaptotagmin Binding to Botulinum Neurotoxins. Biochemistry. 59:491-498.

33. Tao L**, Tian S*, Zhang J*, Liu Z, Robinson-McCarthy L, Miyashita S, Breault D.T., Gerhard R, Oottamasathien S, Whelan S.P.J., Dong M**  (2019) Sulfated glycosaminoglycans and low-density lipoprotein receptor contribute to Clostridium difficile toxin A entry into cells. Nature Microbiology. 4:1760

32. Garland M, Babin BM, Miyashita S, Loscher S, Shen Y, Dong M, Bogyo M (2019). Covalent modifiers of botulinum neurotoxin counteract toxin persistence. ACS Chem Biol. 14:76-87. 

31. Shen Y, Wu SY, Rancic V, Aggarwal A, Qian Y, Miyashita S, Ballanyi K, Campbell RE, Dong M (2019). Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration. Commun. Biol. 2:18 doi: 10.1038/s42003-018-0269-2.

30. Mansfield MJ, Wentz TG, Zhang S, Lee EJ, Dong M**, Sharma SK**, Doxey AC** (2019). Bioinformatic discovery of a toxin family in Chryseobacterium piperi with sequence similarity to botulinum neurotoxins. Scientific Report, 9:1634

29. Elliott M, Favre-Guilmard C, Liu SM, Maignel J, Masuyer G, Beard M, Boone C, Carre D, Kalinichev M, Lezmi S, Mir I, Nicoleau C, Palan S, Perier C, Raban E, Zhang S, Dong M**, Stenmark P**, Krupp J** (2019). Engineered botulinum neurotoxin B with improved binding to human receptors has enhanced efficacy in preclinical models. Science Advances. 5:eaau7196

28. Košenina S, Masuyer G, Zhang S, Dong M*, Stenmark P* (2019). Crystal structure of the catalytic domain of the Weissella oryzae botulinum-like toxinFEBS Lett. 593(12):1403-1410.

27. Tian S, Wu Q, Zhou B, Choi MY, Ding B, Yang W, Dong M (2019). Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex. Cell Rep. 28(10):2517-2526.e5.

26. Dong M, Masuyer G, Stenmark P (2019). Botulinum and tetanus neurotoxins. Annual Review of Biochemistry, 88:811. 

25. Tian S, Muneeruddin K, Choi MY, Tao L, Bhuiyan RH, Ohmi Y, Furukawa K, Furukawa K, Boland S, Shaffer SA, Adam RM, Dong M (2018). Genome-wide CRISPR screens for Shiga toxins and Ricin reveal Golgi proteins critical for glycosylation. PloS Biology16:e2006951

24. Chen P*, Tao L*, Wang T, Zhang J, He A, Lam K, Liu Z, He X, Perry K, Dong M**, Jin R** (2018). Structural basis for recognition of frizzled proteins by Clostridium difficile toxin BScience, 360:664.* co-corresponding authors. 

23. Zhang S, Lebreton F, Mansfield MJ, Miyashita S, Zhang J, Schwartzman JA, Tao L, Masuyer G, Carranza M, Stenmark P, Gilmore MS, Doxey A*, Dong M* (2018). Identification of a botulinum neurotoxin-like toxin in a commensal strain of Enterococcus faecium, Cell Host and Microbe, 23:169-176. * co-corresponding authors. 

22. Masuyer G, Zhang S, Barkho S, Shen Y, Henriksson L, Kosenina S, Dong M*, Stenmark P* (2018). Structural Characterization of the catalytic domain of botulinum neurotoxin X – high activity and unique substrate specificity.  Scientific Reports, 8:4518. * co-corresponding authors.

21. Chevalier A, Silva DA, Rocklin G, Hicks DR, Vergara R, Murapa P, Bernard SM, Zhang L, Lam KH, Yao G, Bahl CD, Miyashita S, Goreshnik I, Fuller JT, Koday MT, Jenkins C, Colvin T, Carter L, Bohn A, Bryan CM, Velasco DA, Stewart L, Dong M, Huang X, Jin R, Wilson IA, Fuller DH, Baker D (2017). Massively parallel de novo protein design for targeted therapeutics. Nature (article), 550:74-79

20. Zhang S, Berntsson R, Tepp WH, Tao L, Johnson EA, Stenmark P*, and Dong M* (2017). Structural basis for the unique ganglioside and cell membrane recognition mechanism of botulinum neurotoxin DC, Nature Communications, 8, 1637. * co-corresponding authors.

19. Huang N, Pishesha N, Mukherjee J, Zhang S, Deshycka R, Sudaryo V, Dong M, Shoemaker CB, Lodish HF (2017). Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin. Nature Communications, 8:423, PMC5583347.

18. Tao L*, Peng L*, Berntsson R, Liu S, Park S, Yu F, Boone C, Palan S, Beard M, Chabrier P, Stenmark P**, Krupp J**, Dong M** (2017). Engineered botulinum neurotoxin B with improved efficacy for targeting human receptors. Nature Communications 8:53. * co-first authors; ** co-corresponding authors. 

17. Zhang S, Masuyer G, Zhang J, Shen Y, Lundin D, Henriksson L, Miyashita S, Martinez-Carranza M, Dong M*, Stenmark P* (2017). Identification and characterization of a novel botulinum neurotoxin. Nature Communications 8:14130. * co-corresponding authors.

16. Tao L, Zhang J, Meraner P, Tovaglier A, Wu X, Gerhard R, Zhang X, Stallcup WB, Miao J, He X, Hurdle JG, Breault DT, Brass AL, Dong M (2016). Frizzled proteins are colonic epithelial receptor for C. difficile toxin BNature (article, 538: 350-355, doi:10.1038/nature19799).

— “Research Highlight” Nature Reviews Microbiology 


15. Yao G*, Zhang S*, Mahrhold S*, Lam K, Stern D, Bagramyan K, Perry K, Kalkum M, Rummel A**, Dong M**, Jin R**(2016). N-linked glycosylation of SV2 is required for binding and uptake of botulinum neurotoxin A. Nature Structure and Molecular Biology, 23, 656-662. * co-first author; ** co-corresponding authors.

 -- “News and Views”   Nature Structure & Molecular Biology, 2016, 23:619-621.

14. Lee K, Zhong X, Gu S, Kruel AM, Dornet MB, Perry K, Rummel A, Dong M, Jin R (2014). Molecular basis for disruption of E-cadherin adhesion by botulinum neurotoxin A complex. Science, 344:1405-10.

13. Peng L, Adler M, Demogines A, Borrell A, Liu H, Tao L, Tepp WH, Zhang SC, Johnson EA, Sawyer SL and Dong M (2014).Widespread sequence variations in VAMP1 across vertebrates suggest a potential selective pressure from botulinum neurotoxins. PLoS Pathogens 10(7):e1004177, PMCID: PMC 4092145.

12. Berntsson RP-A, Peng L, Svensson LM, Dong M, Stenmark P (2013). Crystal Structures of Botulinum Neurotoxin DC in Complex with Its Protein Receptors Synaptotagmin I and II. Structure, 21:1602. PMC3803103.

11. Berntsson RP-A, Peng L, Dong M, Stenmark P (2013). Structure of Dual Receptor Binding to Botulinum Neurotoxin B. Nature Communications, 4:2058. PMC3752466.

10. Peng L, Liu H, Ruan H, Tepp WH, Stoothoff WH, Brown RH, Johnson EA, Yao WD, Zhang SC, and Dong MCytotoxicity of Botulinum Neurotoxins Reveals Essential Neuronal Plasma Membrane SNAREs. Nature Communications2013; 4:1472. 

   -- Harvard News report: http://hms.harvard.edu/news/new-wrinkle-botox-3-26-13

   -- Faculty of 1000 recommended

9. Peng L, Berntsson RP-A, Tepp WH, Pitkin RM, Johnson EA, Stenmark P, and Dong M (2012). Botulinum neurotoxin D-C uses synaptotagmin I/II as receptors and human synaptotagmin II is not an effective receptor for type B, D-C, and G toxins. J. Cell Science,125:3233 (PMCID in progress). 

   — Comments in J. Cell Science (2012), 125:e1306

   — 2012 Roger T. Kelleher Award from the New England Primate Center for outstanding research paper

8. Peng L, Tepp WH., Johnson EA., Dong M (2011). Botulinum neurotoxin D uses synaptic vesicle protein SV2 and gangliosides as receptors. PLoS Pathogen, 7:e1002008. PMCID: PMC3068998

   — 2010 Roger T. Kelleher Award from the New England Primate Center for outstanding research paper


Publications in Edwin Chapman’s lab, University of Wisconsin and HHMI:

7. Dong M, Liu H, Tepp WH, Johnson EA, Janz R, Chapman ER (2008).“Glycosylated SV2A and SV2B mediate the entry of botulinum neurotoxin E into neurons”Mol. Bio. Cell, 19(12):5226  

6. Dong M, Tepp WH, Liu H, Johnson EA, Chapman ER (2007). “Mechanism of BoNT/B and G entry into hippocampal neurons”J. Cell Biol., 179(7):1511 

5. Chai Q*, Arndt JW*, Dong M*, Tepp WH, Johnson EA, Chapman ER, Stevens RC (2006). “Structural basis of cell surface receptor recognition by botulinum neurotoxin B”, Nature, 444 (7122) :1096  (*equal contribution) 

   -- Comments in: Nature (2006), Dec 21;444(7122):1019-20

                             Nature Struct. Mol. Biol. (2007), Jan;14(1):9-10

4. Dong M, Yeh F, Tepp WH, Dean C, Johnson EA, Janz R, Chapman ER (2006). “SV2 is the protein receptor for botulinum neurotoxin A”Science, 312(5773):592 

   -- Comments in: Science (2006), Apr 28;312(5773):540-1“A Neuronal Receptor for Botulinum Toxin”.

                           Nature Reviews Neuroscience (2006), vol.7, pp.420-421“Illicit entry”. 

                           Chemical Biology (2006), vol.1, pp.117..  “Botox on the Brain”.

                           Physiology (2006), vol.21, pp. 229“Highlights

3. Dong M, Tepp WH, Johnson EA, Chapman ER (2004). “Using fluorescent sensors to detect botulinum neurotoxin activity in vitro and in living cells”PNAS (USA), 101(41): 14701 

    -- This study has led to the creation of a biotech company, BioSentinel LLC, to commercialize the inventions. 

2. Dong M, Richards DA, Goodnough MC, Tepp WH, Johnson EA, Chapman ER (2003). “Synaptotagmins I and II mediate entry of botulinum neurotoxin B into cells”J.Cell Biol., 162(7):1293 

    --  Comments inJournal of Cell Biology (2003), vol.162, pp.1178. “How to block Botox”.

1. Lewis JL, Dong M, Earles CA, Chapman ER (2003). “The transmembrane domain of syntaxin 1A is critical for cytoplasmic domain protein-protein interaction”J. Biol. Chem., 276(18):15458

© Dong Lab updated July 2016